Training Years: 2017-2018
Training Site: University of the Witwatersrand (Wits)
Country: South Africa
Mentors: Ian Sanne, FCP(SA), FRCP(lon); Jon Juliano, MD, MSPH
Title: Pilot study of routine use of whole genome sequencing to inform treatment for drug-resistant tuberculosis in Johannesburg, South Africa
Project Objectives:
Aim 1: To compare time to initiation of appropriate therapy in a randomized trial of 50 patients with rifampicin resistant TB who will be divided into two arms: 1) standard of care drug sensitivity testing [DST] (i.e. line probe assays [LPA] and phenotypic DST) vs 2) whole genome sequencing (WGS) in addition to standard of care DST.
Aim 2: To assess the agreement between WGS genotypic DST and gold standard phenotypic DST
Aim 3: To determine the feasibility of routine WGS for DST in DR-TB patients by evaluating test turn-around time and cost as compared to current standard of care DST
NIH Support: Fogarty fellowship postdoctoral training award
